HBV cccDNA of patients with hepatitis B in the serum, PBMC and liver tissue distribution
10.3760/cma.j.issn.1003-9279.2008.05.008
- VernacularTitle:HBVcccDNA在乙型肝炎患者血清、外周单核细胞及肝组织中的分布
- Author:
Yong-Le ZHANG
1
;
Dai XU
;
Xiao-Xing WU
;
Jing WU
;
Xin-Yi SHAO
;
Hong CHEN
;
Qin ZHANG
Author Information
1. 杭州市第六人民医院
- Keywords:
Monocytes;
Liver;
DNA
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):342-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective Hepatitis B virus covalently closed circular DNA in the serum of patients with hepatitis B, peripheral mononuclear cells (PBMC) and liver tissue distribution. Methods Serum HBV DNA105 copies/ml 50 cases of hepatitis B patients, serum HBV DNA105 copies/hal 30 cases of hepatitis B patients, patients with fatty liver (hepatitis B) 20 cases, using real-time quantitative palymerase chain reaction for detection of serum, and liver tissue in PBMC HBVcccDNA the existence. Results Of 50 serum HBV DNA105 copies/ml cccDNA the serum specimens were 28 cases, 56% detection rate, 29 cases were PBMC HBVcccDNA, 58% detection rate, liver tissue HBVcccDNA were 44 cases, 88% detection rate, serum, the PBMC were detected in liver tissue were significant differences in P＜0.005, compared serum PBMC were no significant differences in P ＞0.005. 30 serum HBV DNA105copies/ml the serum specimens, PBMC cccDNA detected two cases were 6.67% detection rate, liver tissue cccDNA were six cases 20% detection rate, serum, PBMC, were among the liver tissue was not significantly different P＞0.005. 20 in the serum of patients with fatty liver, liver tissue in PBMCwere not detected HBVcccDNA. Conclusion HBVcccDNA mainly in the liver of patients with hepatitis B, hepatitis B patients and also cccDNA PBMC in the presence of liver tissue but a few of many.
