Study on the relationship between microsatellite alterations of RASSF1A gene and the development of cervical carcinoma
10.3760/cma.j.issn.1003-9279.2008.05.009
- VernacularTitle:抑癌基因RASSF1A微卫星变异与宫颈癌发生发展的关系
- Author:
Fu-Xi ZHAO
1
;
Jie YAN
;
Run-Hua LIU
;
Xi-Ying WANG
;
Li-Hua LIU
Author Information
1. 大同大学医学院
- Keywords:
Genes;
RASFIA;
Loss of heterozygosity;
Microsatellite repeats;
Cervix neoplasms;
Papillomavirus;
human
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):345-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between micresatellite alterations of RASSF1A gene and the development of cervical carcinoma, and HPV16 infection. Methods Two sites of microsatellite polymorphism of RASSF1A gene were selected, we used polymerase chain reaction(PCR) technique to detect the LOH and MSI of cervical tissues, and to detect the infection state of HPV16. Results There were significant differences of LOH rates at the two sites between clinical stage and pathological grade(P ＜ 0.05). Significant differences were noted between the cervical carcinomas with lymph node metastasis and those without lymph node metastasis in regard to their LOH and MSI at the two sites(P＜0.05). The incidence of LOH of RASSF1A gene was higher in HPV16 (+) than that in HPV16(-) (P＜0.05). Conclusion The change of RASSF1A gene is a relatively late event in cervical carcinomas. The detection of the LOH and MSI of RASSF1A gene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma. Infection of HPV16 and LOH of RASSF1A gene had reacted together in the development of cervical carcinoma.
