Study on relationship between Hepatitis B Virus DNA load and genotype with Large Envelope Protein
10.3760/cma.j.issn.1003-9279.2008.05.010
- VernacularTitle:乙型肝炎病毒基因型和DNA载量与外膜大蛋白关系研究
- Author:
Gao-Feng RAO
1
;
En-Fu CHEN
;
Ming-He YAN
;
Min-Qiao ZHENG
Author Information
1. 温州医学院附属温岭医院
- Keywords:
Hepatitis B virus;
Genotype;
DNA;
Viral load;
Virus envelope proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):348-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein. Methods Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofiuorescence assay in 140 serum samples collected from chronic hepatitis B patients. The genotypes of HBV were identified by DNA sequencing; and analyze their relationship. Results There was no significant difference between positive rate of LHBs and that of HBV DNA in HBeAg negative and positive group(P＞0.05); The HBV LHBs absorbency was markedly correlated with the HBV DNA load(R2=0.9267). The difference of HBV LHBs absorbency between HBV genotype B and C was not significant. Conclusions The close correlation between HBV LHBs absorbence and HBV DNA load illustrated that he level of serum LHBs can be used to estimate the state of HBV replication; and there is no relationship between HBV LHBs absorbency and genotypes. So HBV LHBs may be used as a new serological marker to detect HBV replication.
