High-throughput Detection of Drug-resistance Gene mutations in HBV Using MALDI-TOF Mass Spectrometry
10.3760/cma.j.issn.1003-9279.2008.05.011
- VernacularTitle:应用MALDI-TOF-MS技术高通量检测乙肝病毒耐药基因变异
- Author:
Bo-Ping ZHOU
1
;
Hong-Mei ZHANG
;
Xiao-He LI
;
Jing YUAN
;
Wei LI
;
Liu-Mei XU
;
Huo-Sheng WANG
;
Xin-Chun CHEN
;
Chun-Ming DING
Author Information
1. 深圳市东湖医院
- Keywords:
Hepatitis B Virus;
MALDI-TOF-MS;
DNA;
Seqences analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(5):351-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a high-throughput clinical method on drag-resistance gene mutations of HBV using MALDI-TOF-MS. Method Using MassArray Assay Design software designed the iPLEX primers and followed the iPLEX instruction for amplification, SAP reaction, primer extenction, desalination, dispensing, MALDI-TOF-MS screening and data analysis of the gene mutation locus. 138 serum samples of chronic HBV patients with single drug-resistance or multiple drug-resistance on Lamivudin, adefovi, Entecavir were detected. Result The HBV gene mutation platform was successfully developed and applied on the high-throughput dectection of clinical serum samples. It was also a high throughput assay which could be used to detect for more than 138 samples once. The MALDI-TOF-MS technology and the DNA sequencing simultaneously examine 33 samples, in which result of 10 sample is inconsistent, the including 2 samples by MALDI-TOF-MS technology has not tested, 1 sample has 2 inconsistent mutations. Conclusion Detction of HBV gene mutations using MALDI-TOF-MS is highly-sensitive, highly-accuate, high-thoughput,fast achieved and suithle to use in the diagnosis and monitoring of HBV.
