Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat.
- Author:
Jing DONG
1
;
Jun CHENG
;
Qinhuan WANG
;
Shuangshuang SHI
;
Gang WANG
;
Chongwen SI
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA; genetics; Female; Humans; Liver Regeneration; genetics; Male; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Proteins; genetics; Pseudogenes; genetics; Rats
- From: Chinese Medical Sciences Journal 2002;17(2):63-67
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat.
METHODSPolymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome.
RESULTSA piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified. The lengths of the gene and pseudogene are 1508 bp and 442 bp, respectively. The ALR gene of rat includes 3 exons and 2 introns. The 442 bp DNA sequence may represent a pseudogene or a ALR-related peptide. Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene. ALR-related peptide is 84 amino acid residues in length and relates closely to ALR protein.
CONCLUSIONThere might be a multigene family of ALR in rat.
