The coexpression of the preS1 (1-42) and the core (1-144) antigen of HBV in E. coli.
- Author:
Yangqing ZHAO
1
;
Meiyun ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: DNA Primers; Escherichia coli; metabolism; Gene Expression; Hepatitis B Core Antigens; biosynthesis; genetics; Hepatitis B Surface Antigens; biosynthesis; genetics; Molecular Weight; Polymerase Chain Reaction; Protein Precursors; biosynthesis; genetics; Recombinant Fusion Proteins; biosynthesis; chemistry; genetics
- From: Chinese Medical Sciences Journal 2002;17(2):68-72
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the therapeutic T cell vaccine for the treatment of chronic hepatitis B by improving the cellular immunization of HBsAg vaccine with the coexpression of the preS1 (1-42) and the Core (1-144) antigen of HBV in E. coli.
METHODSThe genes of HBcAg (1-144) and preS1 (1-42) were amplified and fused by PCR. This fused gene was inserted in the prokaryotic expression vector pET-11d and expressed in E. coli.
RESULTSIt was showed by SDS-PAGE that the protein molecular weight of the coexpression product was about 20 kD, 20% of all bacteria protein. The monoclonal antibodies against core and preS1 antibody could react with this fused protein by Western-blot technique respectively. The fused gene was verified by sequencing. Under the immune electron microscopy, this fused protein is typical particle of HBcAg but in an aggregated form.
CONCLUSIONThe results might aid for studying T cell immunotherapeutic vaccine for chronic hepatitis B.
