Effects of TGF beta1 autocrine blockage on osteosarcoma cells.
- Author:
Yong LIU
1
;
Qi-Xin ZHENG
;
Jing-Yuan DU
;
Shu-Hua YANG
;
Zeng-Wu SHAO
;
Bao-Jun XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Antisense Elements (Genetics); biosynthesis; genetics; Autocrine Communication; drug effects; Bone Neoplasms; metabolism; pathology; Cell Division; drug effects; Cell Line, Tumor; Doxorubicin; pharmacology; Humans; Osteosarcoma; metabolism; pathology; RNA, Messenger; genetics; Transfection; Transforming Growth Factor beta; biosynthesis; genetics; Transforming Growth Factor beta1
- From: Chinese Medical Sciences Journal 2004;19(2):155-156
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the effects of transforming growth factor beta1 (TGFbeta1) autocrine blockage on proliferation activity and drug sensitivity of osteosarcoma. METHODS; Northern blot, MTT determination, and 3H thymidine incorporation were used to investigate the effects of antisense TGF beta1 gene on osteosarcoma.
RESULTSThe proliferation of osteosarcoma cells transfected by antisense TGF beta1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased.
CONCLUSIONBlockage of osteosarcoma cells TGF beta1 autocrine loop inhibits cell proliferation and enhances chemotherapy sensitivity.
