Construction and immunogenicity of human papillomavirus type 6b L1 recombinant plasmid.
- Author:
Fang LIU
1
;
Jia-bi WANG
;
Ya-gang ZUO
;
Yue-hua LIU
;
Dong-lai MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; COS Cells; Capsid Proteins; Cercopithecus aethiops; Condylomata Acuminata; immunology; prevention & control; Female; Humans; Immunization; Interferon-gamma; secretion; Interleukin-2; secretion; Interleukin-4; secretion; Mice; Mice, Inbred BALB C; Oncogene Proteins, Viral; biosynthesis; genetics; immunology; Papillomaviridae; genetics; Plasmids; Recombinant Proteins; biosynthesis; genetics; Transfection; Vaccines, DNA; immunology
- From: Chinese Medical Sciences Journal 2004;19(3):233-236
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immunogenicity in mice.
METHODSThe major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic expression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunogenicity study.
RESULTSThe recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice's sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-gamma were increased in the same mice.
CONCLUSIONHPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata.
