Oxidative stress in testicular tissues of rats exposed to cigarette smoke and protective effects of caffeic acid phenethyl ester.
- Author:
Hüseyin OZYURT
1
;
Hidir PEKMEZ
;
Bekir Suha PARLAKTAS
;
Ilter KUS
;
Birsen OZYURT
;
Mustafa SARSILMAZ
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; therapeutic use; Caffeic Acids; therapeutic use; Catalase; metabolism; Glutathione Peroxidase; metabolism; Male; Malondialdehyde; metabolism; Nitric Oxide; metabolism; Oxidative Stress; physiology; Phenylethyl Alcohol; analogs & derivatives; therapeutic use; Rats; Rats, Wistar; Smoking; Superoxide Dismutase; metabolism; Testis; drug effects; physiopathology
- From: Asian Journal of Andrology 2006;8(2):189-193
- CountryChina
- Language:English
-
Abstract:
AIMTo show the oxidative stress after cigarette smoke exposure in rat testis and to evaluate the effects of caffeic acid phenethyl ester (CAPE).
METHODSTwenty-one rats were divided into three groups of seven. Animals in Group I were used as control. Rats in Group II were exposed to cigarette smoke only (4 x 30 min/d) and rats in Group III were exposed to cigarette smoke and received daily intraperitoneal injections of CAPE (10 micromol/kg x d). After 60 days all the rats were killed and the levels of nitric oxide (NO) and anti-oxidant enzymes such as superoxide-dismutase, catalase and glutathione peroxidase (GSH-Px) and the level of malondialdehyde were studied in the testicular tissues of rats with spectrophotometric analysis.
RESULTSThere was a significant increase in catalase and superoxide-dismutase activities in Group II when compared to the controls, but the levels of both decreased after CAPE administration in Group III. GSH-Px activity was decreased in Group II but CAPE caused an elevation in GSH-Px activity in Group III. The difference between the levels of GSH-Px in Group I and Group II was significant, but the difference between groups II and III was not significant. Elevation of malondialdehyde after smoke exposure was significant and CAPE caused a decrease to a level which was not statistically different to the control group. A significantly increased level of NO after exposure to smoke was reversed by CAPE administration and the difference between NO levels in groups I and III was statistically insignificant.
CONCLUSIONExposure to cigarette smoke causes changes in the oxidative enzyme levels in rat testis, but CAPE can reverse these harmful effects.