The expression of Sonic Hedgehog during late bell stage by Western Blot and in situ hybridization.
- Author:
Yan-qiu ZHOU
1
;
Jiu-xiang LIN
;
Hong-ti JIA
Author Information
- Publication Type:Journal Article
- MeSH: Ameloblasts; metabolism; Animals; Blotting, Western; Gene Expression; Hedgehog Proteins; In Situ Hybridization; In Vitro Techniques; Mice; Mice, Inbred BALB C; Tooth Germ; cytology; metabolism; Trans-Activators; biosynthesis; genetics
- From:Chinese Journal of Stomatology 2003;38(3):210-212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and biological effect of Shh during late bell stage by morphological and semi-quantitative analysis.
METHODSTooth germs were selected from new born Bal b/c mouse (P1, P2, P3, P5, P7). Semi-quality of Shh was measured by Western Blot and the expression place and strength of Shh were observed by in situ hybridization.
RESULTSShh was expressed in the ameloblast layer during late bell stage; the expression strength was high in secretive period and decreased with development; the active N-section was detectable before P3.
CONCLUSIONShh expresses specially in the ameloblast layer in late bell stage, and expression quality is related to the function of ameloblasts.
