Gene expression and pathological changes of rat submandibular glands after adenovirus-mediated gene transfer.
- Author:
Xiao-qin WANG
1
;
Song-ling WANG
;
Kai-hua SUN
;
Xiu-qing ZHANG
;
Xiao-yong LIU
;
Chun-mei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; enzymology; genetics; Animals; Gene Expression; Gene Transfer Techniques; Luciferases; genetics; Male; Random Allocation; Rats; Rats, Wistar; Submandibular Gland; pathology; virology
- From: Chinese Journal of Stomatology 2003;38(3):227-229
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate luciferase gene expression and pathological changes of submandibular glands (SMG) of rats after adenovirus-mediated gene transfer.
METHODSAdenovirus-mediated luciferase gene (AdCMVLuc, 10(8) pfu in 50 microl) was injected in to SMG of forty wistar rats. The SMGs were harvested for gene expression measurement and pathological study after 3 days, 1,2,4 and 8 weeks.
RESULTSPeak expression was observed in three days following administration of the vector however, gene expression in submandibular glands decreased rapidly. the pathological changes induced by retrograde injection of AdCMVLuc included: after 3 days to one week, compression of acini, dilation of terminal ducts; after two weeks, slight atrophy of a part of acini, increase of iteracinar distance and focal lymphocyte infiltration in lobules and interlobular ducts; after 4 weeks, recovery evidence was found in acini; after 8 weeks, normal acini and ducts were found. The ultrastructural changes included: 3 days, much more rough endoplasmic reticulum was found both in acini and duct epithelial cell; a lot of mucus drops were found in acini; after 1 week, microvillus decreased in duct epithelial cells, mitochondria increased significantly in acini; intercellular space was enlarged.
CONCLUSIONSAdenovirus-mediated gene transfer can produce biological proteins and induce marked inflammatory changes in rat SMG. The ultrastructural changes suggest high protein synthesis activity in the acinar cells after gene transfer.