The formation of transgenic mice with hemangiomas.
- Author:
Qing XU
1
;
Zhi-yuan ZHANG
;
Wan-tao CHEN
;
Wen-jun YANG
;
Zhu-gang WANG
;
Long WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Polyomavirus Transforming; genetics; physiology; Female; Hemangioma; etiology; Male; Mice; Mice, Transgenic; Microinjections
- From: Chinese Journal of Stomatology 2003;38(5):355-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up the animal model of hemangioma by microinjecting the PyMT transgenic DNA.
METHODSConstructing the transgenic PyMT gene, and microinjecting it into fertilized embryos which were transferred to pseudopregnant recipients then. Observing the phenotype of the newborn-mice, detecting the integration of transgenic DNA by PCR, and analyzing the histological morphon of the neoplasm of the mice.
RESULTSThe transgenic DNA was proved to be right and has been microinjected into 579 fertilized embryos, 62 mice were born. Within the 62 mice, one mouse was found being the phenotype of hemangioma. PyMT gene was expressed in the total DNA of the transgenic mouse by PCR.
CONCLUSIONIt could be a good way to build animal model of hemangioma with transgenic PyMT DNA.
