The study of apoptosis of salivary adenoid cystic carcinoma in nude mice.
- Author:
Jie WANG
1
;
Fu-sheng DONG
;
Qing DONG
;
Hong-tao GU
;
He-xiang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Carcinoma, Adenoid Cystic; chemistry; pathology; therapy; Female; Mice; Mice, Inbred BALB C; Mice, Nude; Proto-Oncogene Proteins; analysis; Proto-Oncogene Proteins c-bcl-2; analysis; Salivary Gland Neoplasms; chemistry; pathology; therapy; Tumor Necrosis Factor-alpha; pharmacology; bcl-2-Associated X Protein
- From: Chinese Journal of Stomatology 2003;38(5):358-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the feature of apoptosis of salivary adenoid cystic carcinoma (SACC) induced by recombined human tumor necrosis factor-alpha (rhTNF-alpha) in nude mice, and to evaluate the related genes expression of apoptosis.
METHODSTwelve SPF grade 4 approximately 5 weeks old female Balb/c nude mice were selected in this study. SACC-83 cells were collected to 6 x 10(7) per milliliter and injected subcutaneously. Group A and B were experimental group which was given 100 x 10(4) IU/kg TNF-alpha or 10 x 10(4) IU/kg TNF-alpha respectively. Group C was only given normal saline and used as normal control. The investigations were adopted by using both light and transmission electron microscope (LM and TEM), flow cytometer and In Situ Cell Death Detection Kit. The evaluations of bax and bcl-2 expression were utilized by immunohistochemistry.
RESULTSThe percentage of apoptosis of transplanted tumors was much higher than that of the control (P<0.01). Apoptotic cells were calcified and grit bodies were formed. Apoptotic cells expressed and contained significantly higher proportions of both bax and bcl-2 proteins (P<0.05).
CONCLUSIONSIt is suggested that calcification may be the obvious feature and the last outcome of the apoptosis of SACC transplanted tumors. Apoptosis induced by TNF-alpha can increase the expressions of bax and bcl-2.
