Polarographic behavior and determination of glimepiride.
- Author:
Huai-ling MA
1
;
Mao-tian XU
;
Peng QU
;
Xiao-hua MA
Author Information
- Publication Type:Journal Article
- MeSH: Catalysis; Humans; Hypoglycemic Agents; analysis; urine; Male; Polarography; methods; Potassium Compounds; analysis; Sulfates; analysis; Sulfonylurea Compounds; analysis; urine
- From: Acta Pharmaceutica Sinica 2005;40(8):750-753
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish a polarographic method of parallel catalytic hydrogen wave for determination of glimepiride.
METHODSThe catalytic wave of glimepiride in the presence of K2S2O8 was used to improve the analytical sensitivity. The rapid determination of glimepiride was done by linear single sweep polarography.
RESULTSThe catalytic hydrogen wave of glimepiride was measured at ca. -1.36 (vs SCE) in 0.09 mol x L(-1) Na2B4O7-KH2PO4 (pH 6.24 +/- 0.1) supporting electrolyte. When 1.0 x 10(-2) mol x L(-1) K2S2O8 was present, the current increased by 25 times, and the peak potentioal was unchanged, producing a more sensitive parallel catalytic hydrogen wave. The peak current of the parallel catalytic hydrogen wave was rectilinear to the glimepiride concentration in the range 1.0 x 10(-7) - 4.2 x 10(-5) mol x L(-1) (r = 0.9990, n = 9). The detection limit was 5.0 x 10(-8) mol x L(-1).
CONCLUSIONThe proposed method could be applied to the determination of glimepiride in pharmaceuticals without preliminary separation.
