Analysis of ephedrine and its metabolites in rat urine by HPLC-ESI-ITMSn.
- Author:
Yong CHEN
1
;
Shao-Lin SHEN
;
Huai-Xia CHEN
;
Feng-Mei HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; methods; Ephedrine; chemistry; metabolism; urine; Male; Molecular Weight; Rats; Rats, Wistar; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; methods
- From: Acta Pharmaceutica Sinica 2005;40(9):838-841
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo estabilish a rapid and sensitive LC-ESI-ITMSn method for the identification of ephedrine and its main metabolites in rat urine.
METHODSAfter optimizing the detection condition of LC-ESI-ITMSn chromatography and mass spectrometry by using a standard ephedrine, the ionization and cleavage rules of ephedrine in ESI-MS and ESI-MSn modes were summarized, and then serving as the basis for the metabolite analysis of ephedrine in rat urine. Rat urine samples of 0-48 h were collected after ig 10 mg x kg(-1) ephedrine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-ESI-ITMSn.
RESULTSThe structures of ephedrine metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, three phase I metabolites and the parent drug ephedrine were identified existing in rat urine, but no phase II metabolites were found.
CONCLUSIONThe LC-ESI-ITMSn method is rapid and highly sensitive and sepecific, it is suitable for the identification of ephedrine and its metabolites in rat urine.
