Establishment of high-throughput drug screening model targeting retinoic acid receptor in cells.
- Author:
Feng GUO
1
;
Yan-Xin LIU
;
De-Xian ZHENG
;
Cheng-Yu JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; administration & dosage; pharmacology; Cell Line; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; methods; Embryo, Mammalian; Genetic Vectors; Green Fluorescent Proteins; genetics; metabolism; Humans; Kidney; cytology; metabolism; Plasmids; Receptors, Retinoic Acid; genetics; metabolism; Recombinant Proteins; genetics; metabolism; Response Elements; genetics; Transfection; Tretinoin; administration & dosage; pharmacology
- From: Acta Pharmaceutica Sinica 2005;40(10):908-911
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo screen new drug for the treatment of acute promyelocytic leukemia, psoriasis and acne, high-throughput drug screening cell models marked by green fluorescent protein (GFP) have been established.
METHODSEight repeats of retinoic acid response element (RARE) were synthesized and cloned into a GFP expression vector. This construct was stably transfected into cells in vitro. Stable and sensitive cell clones with high copy numbers of RARE were selected by retinoic acid (RA) using fluorescence-activated cell sorting (FACS).
RESULTSA cell line has been chosen to be high-throughput drug screening cell model. This model was shown with low background, high sensitive and good reproducibility, and was convenient and inexpensive.
CONCLUSIONThis drug screening cell model can be used for retinoic acid receptor target high-throughput drug screening.
