Effects of dipfluzine on expressions of E-selectin, P-selectin, and ICAM-1 in brain ischemia-reperfusion rats.
- Author:
Guo-hong ZHANG
1
;
Ping LÜ
;
Yong-li WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Ischemia; etiology; Cerebral Cortex; metabolism; pathology; Cinnarizine; administration & dosage; analogs & derivatives; pharmacology; Dose-Response Relationship, Drug; E-Selectin; metabolism; Infarction, Middle Cerebral Artery; complications; Intercellular Adhesion Molecule-1; metabolism; Male; Neuroprotective Agents; administration & dosage; pharmacology; P-Selectin; metabolism; Peroxidase; metabolism; Rats; Rats, Sprague-Dawley; Reperfusion Injury; etiology; metabolism; pathology
- From: Acta Pharmaceutica Sinica 2005;40(12):1091-1095
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo evaluate the effects of dipfluzine on the expressions of E-selectin, P-selectin, and ICAM-1 and the infiltration of polymorphonuclear leukocytes in brain ischemia-reperfusion rats.
METHODSThe model of focal cerebral ischemia was established with the Zea-Longa occluding suture. Dipfluzine (0.25, 0.5 and 1 mg x kg(-1)), flunarizine 0.5 mg x kg(-1) and solvent were injected separately into lingual vein at 30 min after ischemia. The occluding suture was slowly taken away to cause reperfusion at 1 h after ischemia. Rats were decapitated under anesthesia at 24 h after ischemia-reperfusion and brains were immediately removed to do the following procedures. Effects of dipfluzine on morphology of the brain tissue were observed through hematoxylin-eosin (HE) staining. By immunohistochemistry and flow cytometry technique and biochemical method, effects of dipfluzine on P-selectin, E-selectin, ICAM-1 and myeloperoxidase (MPO) were observed.
RESULTSDipfluzine could relieve pathological damages in the brain tissue after ischemia-reperfusion, and reduce the expressions of E-selectin, P-selectin and ICAM-1 and activities of MPO in dose-dependent manner.
CONCLUSIONDipfluzine depresses the expressions of P-selectin, E-selectin, and ICAM-1, which are correlated with their effects on the activities of MPO, suggesting that dipfluzine has anti-inflammation effect in certain extent and could protect brain tissue from ischemia-reperfusion injury.
