Parallel catalytic hydrogen wave of acetylspiramycin caused by dissolved oxygen and its application.
- Author:
Fu-min WANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; administration & dosage; analysis; Catalysis; Hydrogen; Hydrogen-Ion Concentration; Oxygen; chemistry; Polarography; methods; Reproducibility of Results; Spiramycin; administration & dosage; analogs & derivatives; analysis; Tablets
- From: Acta Pharmaceutica Sinica 2005;40(12):1135-1138
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo propose a novel polarographic method for the determination of acetylspiramycin (ASPM) is proposed.
METHODSIn 0.1 mol x L(-1) NH4Cl-NH3 x H2O (pH 8.9) buffer containing dissolved oxygen, ASPM yielded a sensitive parallel catalytic hydrogen wave with the peak potential of -1.63 V (vs SCE) by single sweep polarography.
RESULTSThe 2nd order derivative peak currents (i(p)") of the parallel catalytic hydrogen waves of ASPM showed a linear relationship with its concentrations in the range from 1.74 x 10(-3) microg x mL(-1) to 3.84 microg x mL(-1) (r = 0.9979, n=13). Its detection limit was 5.80 x l0(-4) microg x mL(-1) (3sigma) and RSD (n=13) was 1.24% at the concentration level of 0.871 microg x mL(-1).
CONCLUSIONThe proposed method could be applied to the determination of ASPM in ASPM tablets.
