Chromatographic fingerprint of Dalbergia odorifera and its application in the identification of the crude drugs of different origins.
- Author:
Rong-xia LIU
1
;
Qiao WANG
;
Kai-shun BI
;
De-an GUO
Author Information
- Publication Type:Journal Article
- MeSH: Benzopyrans; chemistry; isolation & purification; China; Chromatography, High Pressure Liquid; methods; Chromones; chemistry; isolation & purification; Dalbergia; chemistry; Flavanones; isolation & purification; Isoflavones; Molecular Structure; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization
- From: Acta Pharmaceutica Sinica 2005;40(11):1008-1012
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish the method of HPLC-fingerprint analysis for the quality control of Dalbergia odorifera and identify its main constituents by HPLC-MS.
METHODSThe 37 hatches of samples were analyzed on a Phenomenex Luna C18 column with a gradient of acetonitrile and 0.3% aqueous acetic acid at a flow rate of 1.0 mL x min(-1) and detected at 275 nm. Furthermore, the typical samples were detected by HPLC-DAD-MS under negative ion mode.
RESULTS37 batches of D. odorifera samples were classified into three types based on the results of similarity analysis. According to the comparison of the tR, MS data and UV maximum absorbance (gamma(max)) values with the standards, 10, 7 and 2 phenolic components were identified in three types of D. odorifera extracts, separately.
CONCLUSIONThe method is repeatable and reliable, and it is capable of effectively controlling the quality of D. odorifera.
