Effect of ginkgolide B on the platelet-activating factor induced changes of chemotaxis and cytoskeleton of macrophages.
- Author:
Shan-ying PENG
1
;
Fu-ying ZHANG
;
Xue-yu OU-YANG
;
Yang LIU
;
Wen-jie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Animals; Chemotaxis, Leukocyte; drug effects; Diterpenes; isolation & purification; pharmacology; Ginkgo biloba; chemistry; Ginkgolides; Lactones; isolation & purification; pharmacology; Macrophages, Peritoneal; metabolism; physiology; Mice; Mice, Inbred C57BL; Plants, Medicinal; chemistry; Platelet Activating Factor; antagonists & inhibitors
- From: Acta Pharmaceutica Sinica 2006;41(2):156-160
- CountryChina
- Language:English
-
Abstract:
AIMTo study the inhibitory effect of ginkgolide B (BN52021) on the PAF induced changes of chemotaxis of murine peritoneal macrophages and the related polymerization of F-actin.
METHODSChemotaxis assays were performed using a modified 48-well Boyden chamber. Actin polymerization of murine peritoneal macrophages was analyzed by flow cytometry using a specific fluorescent stain.
RESULTSPeritoneal macrophages significantly migrated toward platelet-activating factor (PAF) through a micropore filter; however, in the presence of PAF receptor antagonist BN52021 (0.01 nmol x L(-1) -0.1 micromol x L(-1)), the migration was significantly inhibited. Moreover, BN52021 inhibited the actin polymerization of murine peritoneal macrophages induced by PAF in the presence of Ca2+, but not in Ca2+ -free medium.
CONCLUSIONThe results suggested that preventing polymerization of F-actin may be a pathway by BN52021 to inhibit the chemotaxis of macrophages, and this effect seems to be Ca2+ dependent. The data further indicated that inhibition of PAF induced macrophage chemotaxis is an important mechanism underlying the anti-inflammatory action of BN52021.
