Effect of beta-adrenoceptor on NO-induced attenuation in spontaneous contractions of ileum in mice.
- Author:
Li-Qun MA
1
;
Huan-Zhong HU
;
Qin TIAN
;
Chang-Dong WANG
;
Xiao-Min WANG
;
Cheng-Gao YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arginine; pharmacology; Ileum; physiology; Mice; Mice, Inbred Strains; Muscle Contraction; physiology; Nitric Oxide; biosynthesis; Receptors, Adrenergic, beta; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(1):111-115
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the influence of L-arginine (NO donors, L-Arg) on spontaneous contractions of ileum in mice and study the effects of activation of beta-adrenoceptor on NO-induced inhibition in spontaneous contractions of ileum.
METHODSThe method of spontaneous contractions recording was used to investigate the effect of L-NNA, ODQ, Isoprenaline( beta-adrenoceptor agonist) and Propranolol (beta-adrenoceptor antagonist) on NO-induced inhibition in spontaneous contractions of ileum.
RESULTS(1) L-Arg inhibited the spontaneous contractions of ileum and had concentration-response relationship. (2) L-NNA (3 x 10(-4) mol/L), ODQ (3 x 10(-6) mol/L) relieved the inhibitory effect of L-Arg in ileum . (3) Propronalol (3 x 10(-6) mol/L) decreased significantly the inhibitory effect of L-Arg. (4) Iso (1 x 10(-7) mol/L) increased the inhibitory effect of L-Arg. After Iso (1 x 10(-7) mol/L) and Propronalol (3 x 10(-6) mol/L) being coapplied, the inhibitory effect of L-Arg was not changed.
CONCLUSIONNOS catalyzed L-Arg and produced NO. NO exerted its inhibitory effect by the cGMP pathway, the activation of beta-adrenoceptor was partly involved in NO-induced relaxation in ileum.
