Effects of soy isoflavones on the expression of Bax mRNA and Ca(2+)-ATPase activity in ovaries of perimenopause rats.
- Author:
Hai-Xia WEN
1
;
Xiao-Hui XIAO
;
Wei ZHAO
;
Guo-Yi LIU
;
Hong-Zhe SONG
;
Jiang NI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium-Transporting ATPases; metabolism; Female; Isoflavones; pharmacology; Ovary; drug effects; metabolism; Perimenopause; RNA, Messenger; genetics; Rats; Rats, Wistar; Soybeans; chemistry; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(1):117-120
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of soy isoflavones (SI) on the expression of Bax mRNA and Ca(2+) -ATPase activity in ovaries of perimenopause rats.
METHODSThe animal model of perimenopause rats was established by unforced aging. 12 month-old presenilins female Wistar rats were administered by intragastric (ig) with low (500 mg/kg), middle (158 mg/kg) and high (500 mg/kg) does of SI for 8 weeks. The expression of Bax mRNA in ovaries were detected by RT-PCR. Ca(2+) -ATPase activity in ovaries and MDA content and SOD activity in serum were detected by chemi-chromatometry.
RESULTSIntervention of SI could significantly decrease the expression of Bax mRNA in ovaries and MDA content in serum, increase Ca(2+) -ATPase activity in ovaries and SOD activity in serum of presenilins rats (P < 0.05 or P < 0.01).
CONCLUSIONSoy isoflavones could down-regulate the expression of Bax mRNA and increase Ca(2+) -ATPase activity in aged ovaries. It is probably one of the mechanisms to improve the function of aged ovaries in perimenopause rats.
