Effect of ligustrazine and L-arginine on function of mitochondria in myocardium after ischemia/reperfusion injury of myocardium in rabbits.
- Author:
Wan-tie WANG
1
;
Mao-lin HAO
;
Fang-yan WANG
;
Shi-rong NI
;
Zhang-juan SONG
;
Ke-jian XIE
;
Zhou-xi FANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arginine; pharmacology; Calcium; metabolism; Malondialdehyde; analysis; Mitochondria, Heart; drug effects; metabolism; Myocardial Reperfusion Injury; metabolism; physiopathology; Pyrazines; pharmacology; Rabbits; Superoxide Dismutase; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(2):134-137
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of ligustrazine (LGT) and L-arginine(L-Arg)on function of mitochondria in myocardium after myocardial ischemia/reperfusion injury (MI/RI).
METHODS50 rabbits were randomly divided into five groups (n=10): Control group(A), MI/R group(B), MI/R + LGT group (C), MI/R+ L-Arg group (D), MI/R+ LGT + L-Arg group (E). The mitochondrial respiratory function, Ca2+ concentration ([Ca2+]m), malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were deter mined. Meanwhile, the contents of ATP and EC in the myocardial tissue were measured, respectively.
RESULTSIt was found that mitochondrial respiratory control rate (RCR), state 3 (ST3), SOD in C, D, E group were higher than those of B group, state 4 (ST4), [Ca2+]m, MDA were lower than those of B group, ATP and EC levels of myocardial tissue were higher than those in B group; and there were not significant differences between E and A group of above.
CONCLUSIONLGT and IL-Arg can improve function of mitochondria in myocardium after ischemia/reperfusion injury of myocardium in rabbits by decreasing oxygen free radical level and Ca" overload in the mitochondria.
