Dynamics of calcium in the hippocampal neuronal culture model of epilepsy.
- Author:
Jin ZHANG
1
;
Mei-ping DING
;
Zhao LIU
;
Bo XIAO
;
Guo-liang LI
;
Fu-you ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Calcium; metabolism; Cells, Cultured; Hippocampus; cytology; metabolism; Neurons; metabolism; Rats; Rats, Sprague-Dawley; Status Epilepticus; metabolism; physiopathology
- From: Chinese Journal of Applied Physiology 2007;23(2):200-203
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the intracellular calcium ion spatio-temporal and dynamic changes in the hippocampal neuronal culture model of epilepsy induced by low magnesium ion medium, and to explore the relationship between calcium ion and epilepsy.
METHODSApplying both laser scanning confocal microscope and patch clamp to timely observe the changes of [Ca2+]i and electrophysiological in the hippocampal neuronal culture model of epilepsy, and the influence of NMDA receptor-gated channels retarder and non-NMDA receptor-gated channels retarder.
RESULTSAfter the hippocampal nerve cell broken into epileptiform discharges, [(Ca2+]i rapidly ascended to (620 +/- 70) nmol/L, NMIDA acceptor retarder (MK-801, 10 micromol/L) and non-NMDA acceptor retarder (NBQX, 10 micromol/L) reduced [Ca2+]i ascendance. Recovery of the elevated [Ca2+]i was obviously delay, after 90 min and 150 min epileptiform discharges, it took (114.8 +/- 5.2) min and (135.0 +/- 22.7) min (P < 0.05) respectively.
CONCLUSIONIn vitro status epilepticus causes sustained elevation of intracellular calcium levels in hippocampal neurons
