Effect of protooncogene c-myb on progesterone-induced mouse germinal vesicle stage oocyte maturation in vitro.
- Author:
Lei WU
;
Li-ping ZHENG
;
Yue-hui ZHENG
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Genes, myb; Meiosis; Mice; Mice, Inbred Strains; Oocytes; cytology; drug effects; Oogenesis; Progesterone; pharmacology
- From: Chinese Journal of Applied Physiology 2007;23(2):221-226
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of c-myb on progesterone-induced mouse germinal vesicle(GV) stage denuded oocyte (DO) maturation in vitro.
METHODSWe used mouse GV stage oocyte cultured with special concentration progesterone, or/and antisense c-myb ODN, or/and db-cAMP, or/and heparin for 24 h, and observed oocyte maturation and analysed the relationship among them.
RESULTSWe cultured DO in the medium 199 for 24 h, and found 10 micromol/L progesterone had more significant effect than 5 micromol/L progesterone (2 h GVBD% P < 0.05, 8 h PB 1% P < 0.05), but had not more significant effect than 20 micromol/L progesterone. We found that 16 micromol/L antisense c-myb ODN significantly inhibited progesterone (10 micromol/L)-induced mouse germinal vesicle stage oocyte maturation in vitro (2 h GVBD% P < 0.05, 8 h PBI% P < 0.01). 1 x 10(-4) micromol/L dbcAMP, 100 microg/ml heparin could single significantly inhibited progesterone-induced mouse GV stage oocyte maturation in vitro (2 h PBI% all P < 0.01, 8 h PBI% all P < 0.01), and could enhanced the inhibition of 16 micromol/L antisense c-myb ODN (2 h GVBD% all P < 0.01, 8h PBI% all P < 0.01).
CONCLUSIONProgesterone, protooncogene c-myb,cAMP and calcium all pay important role in regulating oocyte maturation and the mechanism of progesterone, cAMP and calcium in regulating oocyte maturation may be through the expression of protooncogene c-myb.