Intrathecal Gabapentin Increases Interleukin-10 Expression and Inhibits Pro-Inflammatory Cytokine in a Rat Model of Neuropathic Pain.
10.3346/jkms.2013.28.2.308
- Author:
Byung Sang LEE
1
;
In Gu JUN
;
Sung Hoon KIM
;
Jong Yeon PARK
Author Information
1. Department of Anesthesiology and Pain Medicine, CHA Medical Center, CHA University, Gumi, Korea.
- Publication Type:Original Article
- Keywords:
Allodynia;
Gabapentin;
Interleukin-10;
Intrathecal;
Pro-inflammatory Cytokines
- MeSH:
Amines/pharmacology/*therapeutic use;
Analgesics/pharmacology/*therapeutic use;
Animals;
Antibodies/immunology/pharmacology;
Behavior, Animal/drug effects;
Cyclohexanecarboxylic Acids/pharmacology/*therapeutic use;
Cytokines/*metabolism;
Disease Models, Animal;
Injections, Spinal;
Interleukin-10/genetics/immunology/*metabolism;
Male;
Neuralgia/*drug therapy/metabolism/pathology;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins/biosynthesis/genetics/pharmacology;
Spinal Cord/metabolism;
Up-Regulation;
gamma-Aminobutyric Acid/pharmacology/*therapeutic use
- From:Journal of Korean Medical Science
2013;28(2):308-314
- CountryRepublic of Korea
- Language:English
-
Abstract:
We examined the possible anti-inflammatory mechanisms of gabapentin in the attenuation of neuropathic pain and the interaction between the anti-allodynic effects of gabapentin and interleukin-10 (IL-10) expression in a rat model of neuropathic pain. The anti-allodynic effect of intrathecal gabapentin was examined over a 7-day period. The anti-allodynic effects of IL-10 was measured, and the effects of anti-IL-10 antibody on the gabapentin were assessed. On day 7, the concentrations of pro-inflammatory cytokines and IL-10 were measured. Gabapentin produced an anti-allodynic effect over the 7-day period, reducing the expression of pro-inflammatory cytokines but increasing the expression of IL-10 (TNF-alpha, 316.0 +/- 69.7 pg/mL vs 88.8 +/- 24.4 pg/mL; IL-1beta, 1,212.9 +/- 104.5 vs 577.4 +/- 97.1 pg/mL; IL-6, 254.0 +/- 64.8 pg/mL vs 125.5 +/- 44.1 pg/mL; IL-10, 532.1 +/- 78.7 pg/mL vs 918.9 +/- 63.1 pg/mL). The suppressive effect of gabapentin on pro-inflammatory cytokine expression was partially blocked by the anti-IL-10 antibody. Expression of pro-inflammatory cytokines was significantly attenuated by daily injections of IL-10. The anti-allodynic effects of gabapentin may be caused by upregulation of IL-10 expression in the spinal cord, which leads to inhibition of the expression of pro-inflammatory cytokines in the spinal cords.
