Protective effect of Naoyikang on the damage induced by glutamate in hippocampal neuron.
- Author:
Ya-E HU
1
;
Ai-Ling ZHOU
;
Yan ZHU
;
Jia-Hui MAO
;
Hai-Yan SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Death; drug effects; Drugs, Chinese Herbal; pharmacology; Glutamic Acid; pharmacology; Hippocampus; cytology; drug effects; metabolism; Neurons; drug effects; metabolism; Neuroprotective Agents; pharmacology; PTEN Phosphohydrolase; metabolism; Rats; Rats, Sprague-Dawley; Serum
- From: Chinese Journal of Applied Physiology 2007;23(3):300-303
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of Naoyikang serum on the damage induced by glutamate in hippocampal neuron.
METHODSMorphological observation, MTT assay and nuclear DNA-associated fluorescence with DAPI dye were applied to evaluate the viability of hippocampal neuron, immunocytochemistry and RT-PCR were used to determine the expression of PTEN.
RESULTSA decreased viability and increased expression of PTEN were shown in hippocampal neuron in response to the treatment with glutamate. It was shown that the percentage of cell death and the expression of PTEN were reduced by the treatment with Naoyikang serum.
CONCLUSIONThese results suggest that Naoyikang may prevent the toxicity of glutamate by suppressing the expression of PTEN.
