Changes of glycine uptake of synaptosomes of rat spinal cord in 0.7 MPa (7ATA) air pressure.
- Author:
Gang BI
1
;
Jian ZHANG
;
Tao YANG
;
Jing-Chang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Air Pressure; Animals; Glycine; metabolism; Rats; Rats, Sprague-Dawley; Spinal Cord; metabolism; Synaptosomes; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(3):334-337
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study changes of function of transmitter glycine in nitrogen narcosis.
METHODSSynaptosomes of rat spinal cord were prepared. Glycine uptake of synaptosomes of rat spinal cord in 0.7 MPa (7ATA) hyperbaric air pressure was observed by the methods of isotope.
RESULTSGlycine uptake slowed down and took a longer period of time to reach saturation in 0.7 MPa (7ATA). The maximum glycine uptake was lessened. Vm was diminished, but Km was increased. Vm rose in 0.7 MPa (7ATA) when corticosterone was added.
CONCLUSIONWhen nitrogen narcosis arose in 0.7 MPa (7ATA), the function of transporters of glycine re-uptake was reduced, the affinity of glycine for transporters subsided. Corticosterone was conductive to the recovery of the function of glycine transporters of high affinity.
