Effects of pravastatin on the expression of endothelin induced by aldosterone in rat cardiac fibroblasts.
- Author:
Yu-Zhou WU
1
;
Wei CUI
;
Shu-Qin LI
;
Lei ZHANG
;
Jing-Chao LU
Author Information
- Publication Type:Journal Article
- MeSH: Aldosterone; metabolism; Animals; Cells, Cultured; Endothelins; metabolism; Fibroblasts; drug effects; metabolism; Myoblasts, Cardiac; drug effects; metabolism; Pravastatin; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2007;23(3):343-346
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of pravastatin on endothelin(ET) expression induced by aldosterone in cultured neonatal rat cardiac fibroblasts.
METHODSET concentration in conditioned medium was measured by radioimmunoassay, intracellular ET-1 level was evaluated by flow cytometry, and the expression of preproendothelin-1 (ppET-1) was detected and quantified using reverse transcriptase-polymerase chain reaction (RT-PCR) method.
RESULTSThe cardiac fibroblasts, treated with aldosterone at 107 mol/L, significantly up-regulated ppET-1 mRNA expression, as well as ET-1 synthesis and release. Pravastatin (10(-5), 10(-4), 10(-3) mol/L) dose-dependently blocked these effects. In contrast, pravastatin-induced inhibitory effects were reversed in the presence of mevalonate.
CONCLUSIONPravastatin down-regulated ppET-1 mRNA expression, as well as ET-1 synthesis and release induced by aldosterone in a process specifically related to mevalonate in cardiac fibroblasts.
