Role of NO signal pathway in the inhibitory of 17beta-estradiol on the production of endothelin-1 in vascular smooth muscle cells.
- Author:
Zhi TAN
1
;
Ting-Huai WANG
;
Yu CHEN
;
Giu-Ping LIN
;
Jing-Yun PAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Endothelin-1; biosynthesis; Estradiol; pharmacology; Muscle, Smooth, Vascular; cytology; drug effects; metabolism; Myocytes, Smooth Muscle; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; drug effects
- From: Chinese Journal of Applied Physiology 2007;23(3):347-350
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the mechanisms of 17beta-estradiol on the production of endothelin-1 in vascular smooth muscle cells.
METHODSAfter incubation VSMC with various concentrations of 17beta-estradiol (10(-9) - 10(-7) mol/L) or plus L-NAME(10(- 6) mol/L) for different times, the concentration of endothelin-1 was measured. At the same time, the activity of endothelin converting enzyme-1 was analyzed, and the expression of preproET-1mRNA was measured by RT-PCR.
RESULTSIn basal conditions, 17beta-estradiol could inhibit the production of endothelin-1 in VSMC, and the action of 17beta-estradiol had nothing to do with the activity of endothelin converting enzyme-1. L-NAME inhibited the effect of 17-estradiol on the production of endothelin-1 in VSMC. RT-PCR results showed that 17-estradiol inhibited the preproET-1 mRNA expression, and whereas L-NAME reversed this action of 17beta-estradiol.
CONCLUSIONIn basal conditions, 17beta-estradiol decreases the preproET-1 mRNA expression through NO-pathway to inhibit the production of endothelin-1 in cultured VSMC.
