Effects of endurance exercise on ERK1/2 phosphorylation and expression in skeletal muscle of rats.
- Author:
Shi-Cheng CAO
1
;
Li-Guang SUN
;
Gang ZHAO
;
Li-Ping YE
;
Huei-Li LIU
;
He ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Glucose Tolerance Test; Insulin; physiology; Male; Mitogen-Activated Protein Kinase 1; metabolism; Muscle, Skeletal; metabolism; Phosphorylation; Physical Conditioning, Animal; physiology; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2007;23(3):351-354
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of exercise on phosphorylated and total ERK1/2, and mRNA of ERK2.
METHODSMale rats were randomly divided into control and trained groups. The trained rats were submitted to 1 h or 1.5 h of exercise daily and had a fragment of their excised gastroenemius muscle, 24 h or 48 h after the last training session. The train lasted for 7 weeks. The changes in the expressions of ERK1/2 and p-ERK1/2 were determined by Western blotting.The expression of ERK2 mRNA was determined by RT-PCR.
RESULTSExercise led to a marked increase in p-ERK1/2 of trained groups as compared with controls, and increased ERK1/2 protein expression of training 1.5 h/d, 24 h and 48 h after the last training session. ERK2 mRNA was increased by exercise 1 h/d, 24 h and exercise 1.5 h/d, 24 h and 48 h after the last training session. Glucose tolerance test found that blood insulin concentration was decreased with exercise training.
CONCLUSIONEndurance exercise could increase muscle responsiveness to insulin by improving the total ERK1/2 and p-ERK1/2, ERK2 mRNA expression.
