The protective effects of group I mGluRs antisense oligonucleotides against neurotoxicity of sodium glutamate on cultured mouse cerebral cortical neurons.
- Author:
Li-ping DONG
1
;
Ming HAN
;
Fang YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Cerebral Cortex; cytology; Mice; Mice, Inbred Strains; Neurons; drug effects; metabolism; Neuroprotective Agents; pharmacology; Oligonucleotides, Antisense; Receptors, Metabotropic Glutamate; genetics; Sodium Glutamate; toxicity
- From: Chinese Journal of Applied Physiology 2007;23(4):438-441
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the protective effect of mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides on impairment of cultured mouse cerebral cortical neurons induced by sodium glutamate (Glu).
METHODSNeuron damage induced by Glu as well as the action of mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides were measured by determining the leakage of lactate dehydrogenase (LDH) from neurons. Immunocytochemistry method was used to detect the expression of anti-mGluRl a and anti-mGluR5. Morphological changes of primary cortical neurons were observed by phase contrast microscope.
RESULTSFollowing the exposure of the cells to 0.1 mmol x L(-1) Glu for 15 min, LDH leakage from neurons increased. mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides(6 or 8 micromol x L(-1)) as well as 50 micromol x L(-1) LY367385 reduced the LDH leakage. mGluR1alpha and mGluR5 immunopositive cells showed in cultured neurons.
CONCLUSIONThe protective effects of mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides on neurons damaged by Glu may relate to antagonizing mGluR1a or mGItlR5.
