Effect of recipient mouse splenocytes taken orally by donor mouse on GVHD after splenocyte transplantation.
- Author:
Bao-An CHEN
1
;
Xiao-Jing DENG
;
Jia-Hua DING
;
Xue-Mei SUN
;
Wei-Min DONG
;
Yan-Zhi BI
;
Yan ZHANG
;
Gang ZHAO
;
Chong GAO
;
Yun-Yu SUN
;
Jun WANG
;
Jian CHENG
;
Yan MA
;
Hui-Hui SONG
;
Wen BAO
;
A SCHMITT
;
M SCHMITT
Author Information
1. Department of Hematology, Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China. bachen@seu.edu.cn
- Publication Type:Journal Article
- MeSH:
Adjuvants, Immunologic;
pharmacology;
Animals;
Cell Extracts;
immunology;
pharmacology;
Cell Transplantation;
Female;
Graft vs Host Disease;
immunology;
prevention & control;
Graft vs Leukemia Effect;
immunology;
Leukemia P388;
therapy;
Lymphocytes;
immunology;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred DBA;
Spleen;
cytology;
immunology;
Whole-Body Irradiation
- From:
Journal of Experimental Hematology
2008;16(1):126-130
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore whether the GVHD in mice can be ameliorated and the GVL effect in mice can be reserved by transfusion of lymphocytes of donors fed with recipient splenocytes effect. Male (DBA-2) mice (H-2(d)) as donors were fed with BALB/c splenocytes, DBA-2 splenocytes, bovine serum albumin, or regular chow, every other day. Induction of tolerance was assessed by a mixed lymphocyte reaction (MLR). Female (BALB/c) mice (H-2(d)) as recipients received total body irradiation (TBI) of 6.0 Gy ((60)Cogamma-ray) followed by inoculation of 3 x 10(3) P388 mouse leukemia cells on the same day. Subsequently, tail vein injection of 2 x 10(7) splenocytes supplied by DBA-2 was undertaken. Control groups were fed identically without leukemia cell inoculation. The results showed that GVHD was significantly ameliorated and CD4(+)/CD8(+) ratio increased in recipient-mice transplanted with splenocytes of tolerated donors, compared with control animals. There was no significant difference in survival rate between different groups of recipients inoculated with leukemia cell. It is concluded that the peroral recipient-mouse splenocytes can ameliorate GVHD without hampering effect on GVL.
