Effect of co-culture of bone marrow mesenchymal stem cells and K562 cells on expression of interleukin 8.
- Author:
Li-Xia WANG
1
;
Hua LU
;
Peng LIU
;
Xiao-Ming FEI
;
Yu-Jie WU
;
Cheng-Ya WANG
;
Jian-Yong LI
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
Coculture Techniques;
Humans;
Interleukin-8;
genetics;
metabolism;
K562 Cells;
Mesenchymal Stromal Cells;
cytology;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2008;16(1):156-158
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of interaction between human bone marrow mesenchymal stem cells (MSCs) and K562 cells on the expression of proangiogenic factor IL-8, the K562 cells were co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium while the controlled K562 cells were cultured alone. The IL-8 gene expression of K562 cells and MSCs was determined by RT-PCR. The results indicated that the expression of IL-8 in K562 cells when co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium was obviously higher than that of K562 cells cultured alone (p<0.01). MSCs co-cultured with K562 cells also had an increased level of IL-8 compared with MSCs cultured alone (p<0.01). It is concluded that the interaction between MSCs and K562 cells via direct contact and the cytokine network promotes expression of IL-8.
