Foxp3-transfected CD4+ CD25- T cells suppress function of dendritic cells.
- Author:
Hao ZHOU
1
;
Wei-Ming LI
;
Ming ZHANG
;
Zheng-Rong LIU
;
Ping ZOU
Author Information
1. Institute of Hematology, Union Hospital, Tongji Medical College, University of Science and Technology, Wuhan 430022, Hubei Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CD4 Antigens;
immunology;
CD4-Positive T-Lymphocytes;
immunology;
Dendritic Cells;
immunology;
Forkhead Transcription Factors;
genetics;
metabolism;
Genetic Vectors;
Immune Tolerance;
genetics;
Interleukin-2 Receptor alpha Subunit;
immunology;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Retroviridae;
T-Lymphocytes, Regulatory;
immunology;
metabolism;
Transfection
- From:
Journal of Experimental Hematology
2008;16(1):164-169
- CountryChina
- Language:Chinese
-
Abstract:
To explore the relationship between expression of Foxp3 gene and immune activity of CD4(+) T cells, the Foxp3 gene was transfected with retroviral vector and applied to forcedly express Foxp3 protein in naive CD4(+)CD25(-) T cells, and then the effect of transfected CD4(+)CD25(-) T cells on immune co-stimulatory molecules and immune function of dendritic cells (DCs) was investigated, and the dependence of direct contact between Foxp3-transfected CD4(+)CD25(-) T cells and DCs was clarified by Transwell test. The results showed that through transfection of retroviral vector, CD4(+)CD25(-) T cells model expressing Foxp3 was established. At 1 week after transfection, proportion of T cells expressing Foxp3 was 38%. CD4(+)CD25(-) T cells forcedly expressing Foxp3 could play immune suppression role in vitro and induce down-regulation of CD80 and CD86 expression on the membrane of DCs. The lymphocyte proliferation test in vitro indicated that Foxp3 transfected CD4(+)CD25(-) T cells could inhibit effect of DCs on activation of allo-lymphocytes. It is concluded that the effect of Foxp3-transfected CD4(+)CD25(-) T cells on DC depends on intercellular direct contact.
