Expression of human ermap gene in umbilical cord blood mononuclear cells during differentiation and development towards erythroid lineage.
- Author:
Li-Dan LIN
1
;
Xin-Rong HE
;
Tie-Zhen YE
;
Ying-Yi HE
;
Jing-Ming GUAN
;
Ying CHEN
;
Jie-Fang LIANG
Author Information
1. Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Blood Group Antigens;
genetics;
metabolism;
Butyrophilins;
Cell Differentiation;
genetics;
Cells, Cultured;
Erythroid Cells;
cytology;
Fetal Blood;
cytology;
Humans;
Leukocytes, Mononuclear;
cytology;
metabolism;
Polymerase Chain Reaction;
methods
- From:
Journal of Experimental Hematology
2008;16(2):328-332
- CountryChina
- Language:Chinese
-
Abstract:
The aim of study was to explore the potential of human erythroid membrane associated protein (ERMAP) gene in erythroid cell differentiation and development, mononuclear cells (MNCs) were isolated from umbilical cord blood and induced to erythroid cell differentiation by SCF, IL-3 and EPO. The cell morphology was observed by using optical microscopy, the positive rate of cells was counted by biphenylamine staining and the ratios of CD36+/CD235a-, CD36+/CD235a+, CD36-/CD235a+ cells were detected by flow cytometry, the change of human ermap gene expression level was analyzed by using fluorescent quantitative PCR (FQ-PCR). The results showed that the ermap gene expression level increased while MNCs were induced to erythroid lineage after treatment with SCF, IL-3 and EPO. It is concluded that the human ermap gene plays an important role in differentiation and development of erythroid cells.
