Biological characteristics of human fetal osteoblastic 1.19 cell line.
- Author:
Wen-Ming CHEN
1
;
Zi-Xing CHEN
;
Jian-Nong CEN
;
Jun HE
;
Xue-Li JIAO
;
Ya-Fang WU
;
Jun ZHANG
;
Qiao-Cheng QIU
;
Lan DAI
Author Information
1. The First Affiliated Hospital, Suzhou University, Jiangsu Institute of Hematology, Suzhou 215006, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
physiology;
Cell Line;
Fetus;
Hematopoiesis;
physiology;
Humans;
Mesenchymal Stromal Cells;
cytology;
physiology;
Models, Biological;
Osteoblasts;
cytology;
physiology
- From:
Journal of Experimental Hematology
2008;16(2):339-344
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the biological characteristics of osteoblasts and their hematopoietic supportive function by using human fetal osteoblastic cell line 1.19 (hFOBs) as a model. The pluripotency markers (Oct-4, Rex-1, hTERT) of hFOBs were analyzed by RT-PCR, the multilineage differentiation experiments were conducted in vitro. Flow cytometry (FCM) was used to identify the surface markers of hFOBs, and RT-PCR was used to analyze their hematopoietic cytokine expression in comparison with bone marrow mesenchymal stem cell (BM-MSC). The results showed that hFOBs expressed several ESC pluripotency markers including Oct-4 and Rex-1, except hTERT. Moreover, hFOBs could also undergo multilineage differentiation into the mesodermal lineages of adipocytic cell types in addition to its predetermined pathway, the mature osteoblast. Both hFOBs and BM-MSC expressed CD44, CD73 (SH3), CD105 (SH2) and CD90 (Thy1), and lack expression of CD34, CD45, or HLA-DR surface molecules. In addition, both hFOBs and BM-MSC expressed SCF, IL-6, and SDF-1alpha mRNA, but only hFOBs could express GM-CSF and G-CSF. It is concluded that human fetal osteoblastic cell line 1.19 may provide a good model to study the osteoblastic regulation role in hematopoiesis in vitro.
