CMV pp65 gene modified dendritic cells activate autologous T cells.
- Author:
Guang-Xun GAO
1
;
Xie-Qun CHEN
;
Jin-Yi ZHANG
;
Hua-Feng ZHU
;
Bao-Xia DONG
;
Hong-Tao GU
;
Ying GAO
;
Yao-Zhu PAN
Author Information
1. Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD;
genetics;
metabolism;
Antigens, Differentiation, T-Lymphocyte;
genetics;
metabolism;
Antigens, Viral;
immunology;
CD4-Positive T-Lymphocytes;
immunology;
CD8-Positive T-Lymphocytes;
immunology;
CpG Islands;
genetics;
Cytomegalovirus;
immunology;
DNA;
genetics;
Dendritic Cells;
cytology;
immunology;
metabolism;
Humans;
Interferon-gamma;
genetics;
metabolism;
Lectins, C-Type;
Lentivirus;
genetics;
metabolism;
Mice;
Phosphoproteins;
genetics;
metabolism;
Viral Matrix Proteins;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2008;16(2):397-400
- CountryChina
- Language:Chinese
-
Abstract:
Cytomegalovirus (CMV) infection is a dangerous complication in patients with chronic graft versus host disease (cGVHD). CMV-specific immunity depends on the activity of T cells. This study was aimed to investigate the effect of CMV pp65 gene modified dendritic cells (DCs) on activation of autologous T cells. Lentivirus system was utilized to introduce the CMV full-length pp65 gene into mouse DCs; CpG-DNA was used to induce mature DCs; flow cytometry and immunofluorescence were used to determine the expression of antigen and IFNgamma in T lymphocytes. The results showed that the DCs were infected with lentivirus at a multiplicity of infection (MOI) of 50 with optimal infectious efficiency of 30%-40%; mature DCs expressing pp65 gene could stimulate autologous naive T cells to express CD69 specifically; mature DCs expressing PP65 could stimulate autologous CD4+ or CD8+ T cells to produce IFNgamma. It is concluded that CMV pp65-modified and CpG-DNA-induced mature DCs can activate CMV-specific T lymphocytes in vitro.
