Analysis on expression and molecular basis for ABO glycosyltransferase with dual specificity.
- Author:
Zhi-Hui DENG
1
;
Hao ZHANG
;
Jian-Qiang ZENG
;
Qiong YU
;
Yu-Qing SU
;
Yan-Lian LIANG
;
Qian LI
Author Information
1. Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China. zhihui_deng@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
genetics;
Asian Continental Ancestry Group;
genetics;
DNA Mutational Analysis;
Erythrocytes;
cytology;
enzymology;
metabolism;
Exons;
genetics;
Glycosyltransferases;
chemistry;
genetics;
Humans
- From:
Journal of Experimental Hematology
2008;16(2):421-424
- CountryChina
- Language:Chinese
-
Abstract:
In order to elucidate the expression and molecular genetic background of ABO gene seven samples with ABO discrepancy further identified as bi-specific ABO gene were studied. All these samples were subjected to phenotyping by monoclonal and polyclonal antisera and were then genotyped by direct DNA sequencing and haplotype-sequencing at the exon 6 and 7 of ABO gene. As a result, six ABO dual-specific alleles were identified in Chinese population. An antigen expressed by these B (A) or Cis-AB individuals varied from very low level to the normal level, compared with common A blood group samples. In conclusion, molecular genetic backgrounds of two pairs out of four samples in all samples were the same, however, the ABO expression showed diverse.
