Ultrastructural analysis of 5' nucleotides distribution in acute myeloid leukemia subtypes.
- Author:
Yong-Xin RU
1
;
Shi-Xuan ZHAO
;
Jin-Hua LIU
;
Yin-Chang MI
;
Xiao-Fan ZHU
;
Tian-Xiang PANG
Author Information
1. Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 30020, China.
- Publication Type:Journal Article
- MeSH:
5'-Nucleotidase;
metabolism;
ultrastructure;
Adolescent;
Adult;
Aged;
Bone Marrow Cells;
cytology;
enzymology;
Child;
Female;
Granulocytes;
enzymology;
Humans;
Leukemia, Myeloid, Acute;
classification;
enzymology;
Male;
Middle Aged;
Young Adult
- From:
Journal of Experimental Hematology
2008;16(3):484-487
- CountryChina
- Language:Chinese
-
Abstract:
5' nucleotides (5'NT), a purine degradative enzyme, is capable of hydrolyzing nucleotide and acting as a phosphotransferase simultaneously. It has critical role in maintaining nucleotide metabolism balance. The present study was aimed to investigate the expression of 5'NT in bone marrow granulocytes (BMGs) from patients with acute myeloid leukemia (AML) and healthy donors comparatively. The BMGs were isolated from bone marrow of 33 patients with AML and 6 healthy donors by using lymphocyte isolating solution. The reactivity of 5'NT was detected by electron microscope and cytochemistry of cytidine monophosphate (CMP). The positive BMG ratio and their index were calculated on the base of ultrastructural observation semiquantitatively. The results indicated that electron microscopy revealed plasma membrane reacting pattern of CMP. Most BMGs from normal donors were CMP negative or exhibited lower active degree. All cases of M(0), M(1), M(2) and t (8; 21) showed high positive percentages and high indexes of BMGs, but no statistic differences between them. APL of t (15; 17) shared lower percentages and indexes than other subtypes. There was no significant difference between APL and normal donors statistically. In conclusions, the results suggested the expression of 5'NT may be associated with BMG differentiation in AML, and APL of t (15; 17) may be a highly differentiated leukemia subtype.
