Influence of interferon alpha on expression of Fas and Fas ligand in dendritic cells from patients with chronic myeloid leukemia.
- Author:
Wen-Li ZHAO
1
;
Yi-Huan CHAI
;
Hai-Long HE
;
Xu-Cang WEI
;
Tong WANG
;
Pei-Ni XING
;
Mei-Sheng LI
Author Information
1. Department of Hematology, The Affiliated Children Hospital of Suzhow University, Suzhou 215003, Jiangsn Province, China. zhaowenli69@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Apoptosis;
drug effects;
Cells, Cultured;
Child;
Culture Media;
pharmacology;
Dendritic Cells;
cytology;
metabolism;
Fas Ligand Protein;
genetics;
metabolism;
Female;
Humans;
Interferon-alpha;
pharmacology;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
metabolism;
pathology;
Male;
Middle Aged;
Philadelphia Chromosome;
Young Adult;
fas Receptor;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2008;16(3):501-505
- CountryChina
- Language:English
-
Abstract:
The study was aimed to investigate the influence of interferon alpha (IFN-alpha) on the expressions of Fas and Fas ligand (FasL) in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to adding stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor alpha (TNF-alpha) and interleukin 4 (IL-4), the IFN-alpha was added to the serum-free medium for DCs. After culturing for 10 - 14 days, cell phenotype and percentage of Ph(1) chromosome were detected by different methods. The expression of Fas or FasL on CML-DCs and cell cycle of DCs labeled with propidium iodine (PI) were measured by flow cytometry. The concentration of sFas in supernatants was analyzed by enzyme-linked immunosorbent assay (ELISA). The results indicated that the expression of co-stimulatory molecules were improved significantly while the percentages of Ph(1) positive cells decreased. The level of Fas on cells was up-regulated and the concentration of sFas decreased. However, the expression of FasL was negative. The ratio of apoptosis rose gradually while the concentration of IFN-alpha increased. It is concluded that IFN-alpha can accelerate the apoptosis of Ph(1) positive cells through Fas/FasL pathway, so the number of Ph(1) negative cells increases relatively.
