Gamma-globin synthesis in K562 cells induced with Tortois plastron, Astragali, Salviae miltiorrhizae and Codonopsis pilosulae.
- Author:
Zhi-Mei GUO
1
;
Hai-Jun LI
;
Xin-Hua QIAN
Author Information
1. Department of Pediatrics, Bethune International Peace Hospital, Shijiazhuang 050082, Hebei Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Astragalus membranaceus;
chemistry;
Codonopsis;
chemistry;
Drugs, Chinese Herbal;
pharmacology;
Female;
Humans;
K562 Cells;
Male;
Materia Medica;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Salvia miltiorrhiza;
chemistry;
gamma-Globins;
biosynthesis
- From:
Journal of Experimental Hematology
2008;16(3):520-524
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effects of tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae on gamma-globin gene synthesis in K562 cells in vitro. Benzidine staining was used to clarify the dose-and time-dependent effects of tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae on hemoglobin synthesis in K562 cells and Western blotting was performed to determine the level of hemoglobin F (alpha(2)gamma(2)). The results indicated that the K562 cells treated with 4 kinds of traditional Chinese medicine had different stain rates of benzidine for: 23.5% (tortois plastron), 19.8% (astragali), 15.8% (salviae miltiorrhizae) and 14.5% (codonopsis pilosulae) at 6 days after the treatment. Western blot indicated that synthesis of HbF increased. It is concluded that tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae enhance globins-gamma synthesis level and increase hemoglobin F level in K562 cells, the effect of which resembles that of sodium butyrate.
