Inhibitive effect of acanthopanax senticosus injection on gentamicin-induced ototoxicity in guinea pigs.
- Author:
Lin-Lin JIA
1
;
Hao TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; metabolism; Eleutherococcus; Gentamicins; toxicity; Guinea Pigs; Hair Cells, Auditory; cytology; drug effects; Plant Extracts; pharmacology
- From: Chinese Journal of Applied Physiology 2006;22(2):246-249
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the antagonistic action of acanthopanax senticosus injection (ASS) on gentamicin ototoxicity.
METHODSGuinea pigs were randomly divided into control group, GM group, ASS group, and ASS + GM group. The changes of hearing threshold, cochlear morphology, expression of caspases-3 were determined by ABR, TEM, and Western blot, respectively.
RESULTSThe ABR threshold in GM group increased markedly. There was no significant difference in ABR threshold between ASS group and control group, but the ABR threshold in ASS group was much lower than that both in GM group and ASS + GM group. Severely injured hair cells with morphological characteristics of apoptosis were found under TEM in GM group, and the hair cells were less injured in ASS + GM group. The results of Western blot showed that the expression of caspase-3 increased markedly in GM group, but it increased slightly in ASS + GM group.
CONCLUSIONASS may antagonize the GM ototoxicity by inhibiting the expression of caspases-3.
