Effect of urotensin II on iNOS expression in human umbilical vein endothelial cells.
- Author:
Dan XU
1
;
Chang-hui LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Human Umbilical Vein Endothelial Cells; drug effects; metabolism; Humans; Nitric Oxide; metabolism; Nitric Oxide Synthase Type II; metabolism; RNA, Messenger; genetics; Urotensins; pharmacology
- From: Chinese Journal of Applied Physiology 2006;22(3):288-291
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the direct effect of urotensin II (U II) on the release of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) mRNA in human umbilical vein endothelial cells (HUVEC).
METHODSHUVEC were cultured with different concentrations of U II (10(-9)-10(-7) mol/L) for 24 hours. Then the supernatant was collected to detect the level of NO and the activity of iNOS, the expression of iNOS mRNA of HUVEC was measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).
RESULTSIn comparison with controls, the level of NO, the activity of iNOS and the iNOS mRNA expression increased significantly (P < 0.05).
CONCLUSIONU II may up-regulate the expression of iNOS mRNA and increase NO generation in HUVEC, it suggests that U II may relax blood vessel by activating iNOS/NO pathway.
