Effects of scopolamine on morphine-dependence in mice and the relationship between the effect and hippocampus intracellular calcium.
- Author:
Li-guang WANG
1
;
Xin-hua GUO
;
Ling-Yun LIU
;
Bai-ying PENG
;
Yu-ning LIU
;
Jun-hong QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Hippocampus; cytology; drug effects; metabolism; Mice; Mice, Inbred Strains; Morphine; pharmacology; Morphine Dependence; metabolism; Scopolamine Hydrobromide; pharmacology
- From: Chinese Journal of Applied Physiology 2006;22(3):307-309
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the effect and mechanism of scopolamine on morphine(Mor)-induced mice dependence.
METHODSThe Mor-dependent mice model was established by intraperitoneal (ip) administered Mor for seven days. Pain threshold, times of jump and hippocampus intracellular free calcium ion concentration ([Ca2+]i) were determined by the heat plate test, naloxone (Nal)-precipitated jumping response and flow cytometry, respectively.
RESULTSThe pain threshold of Mor-dependent mice decreased significantly while there was a marked increase in times of jump, the rate of jumping animals and hippocampus [Ca2+]i. Co-administered scopolamine, the pain threshold of Mor-dependent mice increased significantly; the number of jump, the rate of jumping animals and hippocampus [Ca2+]i all decreased significantly.
CONCLUSIONScopolamine could antagonize the Mor-induced mice dependence, which could be related to decreasing the levels of brain intracellular free calcium.
