Redox modulation of large conductance calcium-activated potassium channels in rat cultured trigeminal ganglion neurons.
- Author:
Zhong-Ling ZHU
1
;
Fang WANG
;
Zhou-Huan WU
;
Li-Hong LONG
;
You JIN
;
Jian-Guo CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Large-Conductance Calcium-Activated Potassium Channels; physiology; Male; Neurons; metabolism; physiology; Oxidation-Reduction; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Trigeminal Ganglion; cytology; physiology
- From: Chinese Journal of Applied Physiology 2006;22(4):390-393
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe redox modulation of ion channel in trigeminal ganglion neurons by oxidants and reducing agents.
METHODSThe effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique.
RESULTSMethionine-specific oxidant chloramine-T (Ch-T) 1 mmol/L slightly increased the current amplitude and this enhancement did not antagonized by DTT. In contrast, cysteine-specific reagent 5, 5'-dithio-bis(2-nitrobenzoic acid) (DTNB) 500 micromol/L significantly decreased current amplitude of BK(Ca) channels. The effect was reversed by the reducing agent 2 mmol/L 1, 4-dithio-DL-threitol (DTT).
CONCLUSIONReactive oxygen species were definitely involved in regulation of native neuronal function via redox modulation of BK(Ca) channels, which are suggested to play compensatory roles under oxidative stress-related conditions.
