Effects of zinc on the expression of metallothionein isoforms in stressed hippocampal neurons in vitro.
- Author:
Wei-Qiang CHEN
1
;
Yi-Yong CHENG
;
Shu-Tian LI
;
Yan HONG
;
Yue HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Hippocampus; cytology; metabolism; In Vitro Techniques; Metallothionein; metabolism; Neurons; metabolism; RNA, Messenger; Rats; Zinc; pharmacology
- From: Chinese Journal of Applied Physiology 2006;22(4):415-418
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo evaluate the effects of different doses of zinc on the expression of metallothionein isoforms in stressed hippocampal neurons in vitro.
METHODSThe cell stress model was developed by corticosterone. The cultured hippocampal neurons were assigned to seven groups as follows: control group, zinc deficiency group, and their corresponding stressed groups, as well as three different levels of zinc complementarity groups.
RESULTSIn zinc deficiency group, the expressions of metallothionein and MT-1 mRNA, MT-3 mRNA were downregulated. On the other hand, inductions of metallothionein and it's mRNAs in stressed zinc complementarity group were increased. In addition, the levels of supernatant IL-6 and NO were increased clearly in zinc deficiency group and corticosterone stressed groups.
CONCLUSIONOur results suggest that zinc deficiency may decrease while zinc complementarity increase the expressions of metallothioneins and MT-1 mRNA, MT-3 mRNA in stressed hippocampal neurons in vitro.
