The effect of hypoxia preconditioning no binding activity of HIF-1 on the HRE with EPO in the hippocampus of mice.
- Author:
Guo SHAO
1
;
Wei-Hua ZHOU
;
Cui-Ying GAO
;
Ran ZHANG
;
Guo-Wei LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Erythropoietin; metabolism; Hippocampus; metabolism; Hypoxia; metabolism; Hypoxia-Inducible Factor 1; metabolism; Male; Mice; Mice, Inbred BALB C; Response Elements
- From: Chinese Journal of Applied Physiology 2007;23(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe change of binding activity of HIF-1 with erythropoietin (EPO) hypoxia response element (HRE) in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning.
METHODSThe hippocampus was removed from mice exposed to hypoxia for 0 run (control group), 1 run (H1 group) and 4 runs(H4 group). Electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP)and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO.
RESULTSBoth in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4.
CONCLUSIONThe increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.
