Effects of intrathecal injection of U0126 on the expression of phospho-CREB in spinal cord of morphine-induced withdrawal rats.
- Author:
Hai-Lin LIU
1
;
Jun-Li CAO
;
Pei-Jing DAI
;
Guo-Long ZHENG
;
Ying-Ming ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Butadienes; pharmacology; therapeutic use; Cyclic AMP Response Element-Binding Protein; metabolism; Injections, Spinal; Male; Morphine Dependence; drug therapy; metabolism; Nitriles; pharmacology; therapeutic use; Protein Kinase Inhibitors; pharmacology; therapeutic use; Rats; Rats, Sprague-Dawley; Spinal Cord; drug effects; metabolism; Substance Withdrawal Syndrome; drug therapy; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore effects of intrathecal injection of U0126 on morphine withdrawal response and the spinal Phospho-CREB expression in morphine-induced withdrawal rats.
METHODSAll the rats were divided into 5 groups: control group, dependence group, withdrawal group, U0126 group (5 microg, it) and DMSO group. Morphine withdrawal score, touch evoked agitation scores(TEA score), immunohistochemical and Western-blotting technique were used to evaluate morphine withdrawal response and the expression of Phospho-CREB in the spinal cord.
RESULTSIntrathecal injection of MEK inhibitor U0126 significantly alleviated morphine withdrawal symptoms. Morphine withdrawal scores in U0126 group (22.5 +/- 4.09) were significantly lower than that of withdrawal group (28.6 +/- 4.89, P < 0.05). TEA score of withdrawal group was 13.5 +/- 2.55, which was significantly higher than that of U0126 group (10.0 +/- 2.76, P < 0.05). Phospho-CREB positive neurons in the spinal dorsal horn of withdrawal group were 380 +/- 71, which is higher than that of U0126 group (293 +/- 47, P < 0.05). Compared with withdrawal group, level of Phospho-CREB protein detected by Western blot in spinal cord of U0126 group was significantly lower.
CONCLUSIONMEK inhibitors U0126 could suppress expression of Phospho-CREB in the spinal cord.
