Effect of activation of mitochondrial ATP sensitive potassium channel and calcium activated potassium channel on the permeability transition of mitochondria from both normal and ischemic rat brain.
- Author:
Fang SHEN
1
;
Li-Ping WU
;
Wei-Guo LIU
;
Yan LU
;
Hua-Wei LIANG
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzimidazoles; pharmacology; Brain; drug effects; metabolism; Brain Ischemia; metabolism; Cell Membrane Permeability; Diazoxide; pharmacology; KATP Channels; metabolism; Male; Mitochondria; drug effects; metabolism; Mitochondrial Membrane Transport Proteins; drug effects; Potassium Channels, Calcium-Activated; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2007;23(1):14-18
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo clarify whether the activation of mitochondrial ATP sensitive potassium channel and calcium activated potassium channel can influence the permeability transition of normal and ischemic brain mitochondria.
METHODSspectrophotometry was used to determine the effect of the two mitochondrial potassium channel agonists on the swelling of normal and ischemic brain mitochondria respectively.
RESULTSIn normal mitochondria, diazoxide and NS1619 could inhibit the decrease of calcium induced mitochondrial absorbance at 520 nm (A520), which were blocked by atractyloside. When compared with the normal mitochondria, mitochondrial A520 decrease in ischemic brain was even more rapid. Diazoxide and NS1619 could still inhibit the calcium induced mitochondrial A520 decrease, which were blocked by atractyloside.
CONCLUSIONActivation of mitochondrial ATP sensitive potassium channel and calcium activated potassium channel can protect brain mitochondria in vitro probably via influencing the mitochondrial permeability transition.
